April 05, 2006
Maiysha Jones presents her Master’s Thesis final oral defense on Thursday, April 6th at 3:00pm in 331 Rosenau Hall. Full details follow.Effect of Incubation Conditions on the Enrichment of Pyrene Degrading Bacteria Identified By Stable Isotope Probing in a PAH Contaminated Soil

Many high molecular weight PAH are known or suspected carcinogens and as ubiquitous environmental pollutants, their remediation is necessary to reduce human and environmental health risk. Soil bioremediation techniques include in situ methods or excavation followed by bioreactor treatment. To determine whether bacterial community diversity depends on treatment method, microcosms of either continuously mixed slurry or static, field-wet soil were examined by stable isotope probing (SIP) with [U-13C]pyrene in an aged PAH-contaminated soil. Recovered 13C-enriched DNA was analyzed by denaturing-gradient gel electrophoresis (DGGE) and 16S rRNA gene clone libraries. The majority of sequences recovered were most closely related to gamma proteobacteria across both treatment methods, however some sequences related to alpha proteobacteria were recovered from the slurry treatment only. The minimal diversity observed across both incubation conditions suggests that pyrene-degrading bacterial community diversity may be independent of treatment method.

Committee Members:
Dr. Frederic K. Pfaender, Advisor
Dr. Michael D. Aitken, Reader
Dr. Stephen C. Whalen, Reader

For further information please contact Rebecca Riggsbee Lloyd by email at Rebecca_Lloyd@unc.edu

 

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