Jun Nakamura, PhD, DVM
Dr. Nakamura’s interests focus on investigating mechanisms of DNA damage response to endogenous and exogenous reactive agents. The reverse genetic approach provides a powerful method for the study of gene function and regulation. DT40 cells originated from a chicken B-lymphocyte line and their knockout mutants are observed to show a strong phenotypic resemblance to murine mutants. Using a series of isogenic DT40 knockout mutants with high-throughput format, he determined that cells deficient in the FANC/BRCA pathway and homologous recombination are hypersensitive to formaldehyde at concentrations found in human plasma. Until that time, formaldehyde had been determined to cause DNA-protein crosslinks but little was known about how such lesions are repaired. Dr. Nakamura's results suggest that the use of syngeneic mutant cell lines, which is a very modern technology, is capable of deciphering the physical causes of DNA damage that are missed by more classical technologies. Indeed, the use of syngeneic lines to the analytic ability of toxicology is worthy of interest to more than simply the DNA repair field. Dr. Nakamura is also interested in synthetic lethality caused by poly(ADPribose)polymerase inhibitors and their translational research.
Honors and AwardsEdward Kidder Graham Teaching Award
2003, UNC-CHYoung Investigator Travel Award
2000, Aspen Cancer ConferenceBernard G. Greenberg Best Dissertation Award
2000, UNC School of Public HealthDissertation Award for Population Studies
1999, Lineberger Comprehensive Cancer CenterTravel Award
1998, American Association for Cancer ResearchYoung Investigator's Award
1997, American Association for Cancer Research
Bioavailability of (geno)toxic contaminants in polycyclic aromatic hydrocarbon-contaminated soil before and after biological treatment. Alden Adrion, Michael Aitken, Jing Hu, Jun Nakamura, Damian Shea (2014). Environmental Engineering Science, 31(4), 176-182.
Molecular dosimetry of endogenous and exogenous O6-Methyl-dG and N7-methyl-G adducts following low dose [D3]-methylnitrosourea exposures in cultured human cells. Jean Clement, Leonard Collins, Jun Nakamura, Vyom Sharma, James Swenberg, Zhenfa Zhang (2014). Chemical Research in Toxicology, 27(4), 480-482.
The endogenous exposome. Wanda Bodnar, Leonard Collins, Yongquan Lai, Kun Lu, Benjamin Moeller, Esra Mutlu, Jun Nakamura, Vyom Sharma, James Swenberg, Rui Yu (2014). DNA Repair.
The endogenous exposome. Wanda Bodnar, Leonard Collins, Yongquan Lai, Kun Lu, Benjamin Moeller, Esra Mutlu, Jun Nakamura, Vyom Sharma, James Swenberg, Rui Yu (2014). DNA Repair, 19.
A neutral glyoxal gel electrophoresis method for the detection and semi-quantitation of DNA single-strand breaks. Jun Nakamura, Brian Pachkowski (2013). Methods in Molecular Biology, 1054.
PhD, Environmental Health Sciences, University of North Carolina at Chapel Hill, 1999
DVM, Veterinary Medicine, University of Osaka Prefecture, 1984
PhD, Veterinary Science, University of Osaka Prefecture, 1984
BS, Veterinary Science, University of Osaka Prefecture, 1982