Nutritional Biochemistry and Molecular Biology

The Nutritional Biochemistry and Molecular Biology Core provides assistance for nutritional biochemical and molecular biological techniques and capabilities. Services include:

Standard Molecular Biology Techniques includes RT, RT-PCR, qRT-PCR, DNA & RNA Isolation/Purification, Primer & Probe Design, Serum Separation, Plasma Separation, Lymphocytes Separation and Virus Isolation.

Basic Molecular Biology Assays include Western Blots, Probe Preparation, In Situ Hybridization, RT-PCR In Situ Hybridization and Protein Estimation by BCA Method.

Oxidative Stress includes Catalase, Glutathione Peroxidase (GPx), Glutathione Reductase (GR), Glutathione S-Transferases (GST), Superoxide Dismutase, Thiobarbituric Acid Substances (TBARS), Oxidized and Reduced Glutathione (GSH/GSSG) and Thioredoxin Reductase.

Inflammation (ELISA OR RIA) includes Acute Phase Proteins, Adhesion Molecules, C-Reactive Proteins, E-Selection, VCAM-1 and ICAM-1.

Adipokines and Cytokines includes Adiponectin, Interleukin-1 & Interleukin-1 HS, Interleukin-2, Interleukin-6 Interleukin-6 HS and TNF-α & TNF-α HS.

Peptide Hormones includes Ghrelin, Insulin, C-Peptide, Leptin and Prolactin.

Nutrients includes Vitamin C, Vitamin E, Ferritin, Magnesium, Glucose and Glycogen.

Drug Abuse Tests includes Amphetamines, Barbiturates, Benzodiazepine, Cannabinoids and Cocaine Metabolites.

Lipids includes B-Hydroxybutyrate, Cholesterol and Triglycerides.

Lipoproteins includes High-Density Lipoproteins (HDL), Low-Density Lipoproteins (LDL), Apolipoprotein A1, and Apolipoprotein B.

Other Assays/Measurements that can be performed include (but are not limited to) Albumin, Aspartate Aminotransferase, DHEA, DHEA-Sulfate, Hepatocyte Growth Factor, Plasminogen Activator Inhibitor-1, Resistin, Sex Hormone Binding Globulin, Total Estradiol, Total Estrone, Phantom Solutions, IGF-BP3, IGF1 Alanine Transaminase, Amino Acids, Alkaline Phosphatase, Total Testosterone and Urinalysis.

Flow Cytometry is a very powerful tool that can be used on small amounts of blood samples. This technique is ideal for clinical nutrition studies in which immune cell subsets might be altered, for large epidemiological studies in which a biomarker can be measured in a small blood sample, in studies of oxidative stress in which oxidative bursts can be monitored, or for measuring intracellular cytokine levels. The core provides use of the equipment and data analysis for the Accuri C6 instrumentation.


Core Personnel

GGG_beck_melinda_01Melinda Beck, PhD, Core Director

Professor and Associate Department Chair
Department of Nutrition 
T: (919) 966-6809
E: melinda_beck@unc.edu

 

Qing Shi, Research Specialist

Department of Nutrition
T: (919) 966-7162
E: qing_shi@unc.edu


Service Request Form
To request nutritional biochemistry and molecular biology services, please complete the online service request form. For more information about the core, please contact Qing Shi, Research Specialist.