Michigan Regional Comprehensive Metabolomics Resource Core (MRC2)

The Michigan Regional Comprehensive Metabolomics Resource Core (MRC2) offers a wide variety of services to assist investigators at every step of metabolomics research, from study design, sample collection, data analysis and interpretation of data.  Services include well developed targeted and untargeted metabolomics platforms, lipidomics, and fluxomics, as well as statistical and bioinformatics tools to for data analysis  and visualization. 


Services

Targeted Metabolomics Laboratory
The Targeted Metabolomics Laboratory provides assays for specific metabolites as requested by individual researchers. The laboratory works with a wide range of sample types, including but not limited to: cells, tissues, and biological fluids from human subjects, research animals, and other organisms. Standard assays offered by the directed metabolomics laboratory include:

  • Metabolite extraction
  • Glycolytic, tricarboxylic acid (TCA) cycle, pentose phosphate pathway and nucleotide metabolites
  • Amino Acid Analysis
  • Bile Acids, Oxysterols, and Phytosterols
  • Vitamins
  • Neurotransmitters
  • Carbohydrates and Alditols in bowel
  • NAD-related metabolome
  • Fluxomics


Untargeted Metabolomics Laboratory

The Untargeted Metabolomics Laboratory provides untargeted profiles of a significant number of metabolites in a variety of tissues, serum, plasma and cultured cells using a highly automated workflow. The Untargeted Metabolomics platform is based on four interwoven and fully integrated aspects:

  • Hardware: Because of its high mass resolution and mass accuracy, our platforms are based on HPLC/Q-TOF technology. The current generation of mass specs and software are intensely powerful, but NMR, FTIR, and other systems are available when mass spectrometry is not sufficient.
  • Informatics: The science of Metabolomics is driven at least as much the informatics infrastructure as by the actual hardware platform. This begins with a LIMS system capable of tracking samples, the process through data acquisition and analysis, to the final report. A separate set of tools is available for quality control, initial data analysis and report generation.
  • Robotics: Humans are too good at thinking and being creative to do the repetitive, mundane, error prone tasks involved in most sample handling. Automating processes wherever possible reduces error and enhances data quality.
  • Standards-Based QA/QC: The use of standards to monitor all aspects of the sample preparation and analytics assures a continuously improved process in which the level of error is always known. This allows better experiment design, which yields better quality information from fewer samples.


Standard Assays Offered by the Untargeted Metabolomics Laboratory:

  • HILIC- and Reversed phase based LC/MS
  • GC/MS

Project Requests
UNC research base members will need to complete a service agreement. After an initial consultation, an ‘Exhibit A’ will be drafted by the Core that specifies the scope and total costs for your project, and is part of the MC service agreement.  A copy of U-M’s W9 form can be found below.  These documents should be forwarded to the appropriate authority at UNC. A PO number and copies of the signed contract must be e-mailed to the Core Administrator before services can be initiated.


For more information, please visit the Michigan Regional Comprehensive Metabolomics Resource Core web site or contact:

Stephen C. Brown, PhD
Program Manager
University of Michigan - Metabolomics Core
T: (734) 232-0842
F: (734) 232-0812
E: stephecb@umich.edu

* Please inform Dr. Brown that you are a member of the UNC NORC research base.

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