Atomic Absorption Spectrometry Lab

The Atomic Absorption Spectroscopy Lab provides analysis that is used to determine the levels of arsenate, methylated and other organic forms of arsenic in solutions and/or matrices. Services include:

Oxidation state-specific analysis of arsenic (aqueous solutions or simple biological matrices)
Trivalent and pentavalent arsenic species in aqueous solutions or simple biological matrices (e.g., cell culture medium or urine) are analyzed directly (without extraction or chemical digestion) using hydride generation-cryotrapping-atomic absorption spectrometry (HG-CT-AAS). This method detects and quantifies 7 arsenic species, including arsenite, arsenate, methylarsenite, methylarsenate, dimethylarsenite, dimethylarsenate, and trimethylarsine oxide.

Oxidation state-specific speciation analysis of arsenic (aqueous solutions or simple biological matrices) with very low arsenic content
Trivalent and pentavalent arsenic species in aqueous solutions or simple biological matrices (e.g., cell culture medium or urine) are analyzed directly (without extraction or chemical digestion) using HG-CT-inductively coupled with the plasma-mass spectrometer (ICP-MS). This method can detect and quantify very low levels of 7 arsenic species, including arsenite, arsenate, methylarsenite, methylarsenate, dimethylarsenite, dimethylarsenate, and trimethylarsine oxide.

Analysis of total arsenic or selenium (aqueous solutions or simple biological matrices)
Total arsenic or selenium in aqueous solutions or simple biological matrices (e.g., cell culture medium or urine) is analyzed directly (without extraction or chemical digestion) using HG-CT-AAS or graphite furnace (GF)-AAS, providing only information about the total amount of the element.

Analysis of total arsenic or selenium (complex biological matrices)
Total arsenic or selenium in complex biological matrices (e.g., sediments, cells or tissues) is analyzed after extraction or chemical digestion of samples, using HG-CT-AAS or graphite furnace (GF)-AAS and providing only information about the total amount of the element.

Speciation analysis of arsenic (complex biological matrices)
Arsenic species in complex biological matrices (e.g., sediments, cells or tissues) are analyzed after extraction or chemical digestion of samples using HG-CT-AAS. This method detects and quantifies inorganic arsenic, methylarsenic, dimethylarsenic and trimethylarsenic without providing information on the oxidation state of arsenic in the above species.

Speciation analysis of arsenic (aqueous solutions or simple biological matrices)
Arsenic species in aqueous solutions or simple biological matrices (e.g., cell culture medium or urine) are analyzed directly (without extraction or chemical digestion) using HG-CT-AAS. This method detects and quantifies inorganic arsenic, methylarsenic, dimethylarsenic and trimethylarsenic without providing information on the oxidation state of arsenic in the above species.  

Speciation analysis of arsenic (aqueous solutions or simple biological matrices) with very low arsenic content
Arsenic species in aqueous solutions or simple biological matrices (e.g., cell culture medium or urine) are analyzed directly (without extraction or chemical digestion) using HG-CT-inductively coupled with the plasma-mass spectrometer (ICP-MS). This method can detect and quantify very low levels of inorganic arsenic, methylarsenic, dimethylarsenic and trimethylarsenic without providing information on the oxidation state of arsenic in the above species.


Lab Personnel

GGG_styblo_miroslav2Mirek Styblo, PhD, Lab Director

Associate Professor
Departments of Nutrition and Environmental Sciences & Engineering
T: (919) 966-5721
E: styblo@med.unc.edu


 Service Request Form

To request atomic absorption spectroscopy lab services, please complete the online service request form. For more information about the lab, please contact Mirek Styblo, PhD, Lab Director.