Molecular Biology and Nutritional Biochemistry Core Facility is now offering flow cytometry services. Similar to a fluorescent microscope, the flow cytometer can identifycells by specific cell surfacemarkers or by labeling of internalproteins.

Dr. Patricia Sheidan and Erik Karlsson

Dr. Patricia Sheidan and Erik Karlsson

However, unlike microscopy, the flow cytometer allows forhigh‐throughout capabilities. Simultaneous multi parametric analysis of physical and chemical properties of cells can also be detected and quantified. Flow cyotmetry is a very powerful tool and can be used on small amounts of blood samples. This technique is ideal for clinical nutrition studies:

• In which immune cell subsets might be altered

• For large epidemiological studies in which a biomarker can be measured in a small blood sample

• In studies of oxidative stress in which oxidative bursts can be monitored,

• For measuring intracellular cytokine levels, etc. 

Services are offered at the following levels:  

The Core facility does all the preparing and running of samples ‐ This would require users to provide the samples only; the rest of the analysis is performed by the Core. 

The Core users “rent” time on the machine ‐ In this case, the user would do all the sample preparation. Once trained, they would be allowed to run their own samples on the flow cytometer.

Use of flow cytometer offsite ‐ For brief periods of time, users will be allowed to move the equipment to study sites if shipping of blood samples is impractical. 

• Under all conditions, the CNRC core director and technician are available for consultation in designing experimental protocols as well as data interpretation. To request flow cytometry services, please complete the online service request form at http://www.sph.unc.edu/cnrc/core_c.html. 

Qing Shi

Qing Shi

To learn more about flow cytometry services,
please contact research technician,

Qing Shi at qing_shi@unc.edu

 

 

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